Haejo Jeong

Project: "Characterization and Selection of simpk4 Mutants via Root Phenotype Analysis Using Segregation and Molecular Techniques"

• Performed segregation and Chi-square tests to analyze inheritance patterns
• Executed genomic DNA extraction, PCR amplification, and gel electrophoresis to validate target regions containing the mutation
• Conducted Sanger sequencing and sequence analysis using UGENE to identify a mutation in exon
• Utilized RStudio and ImageJ for analysis and data visualization

Molecular Biology Practical

• Hands-on training in PCR, ELISA, protein extraction, DNA extraction, SDS-PAGE, GUS assay, and other molecular biology techniques
• Conducted Gibson Assembly cloning to assemble DNA fragments and construct recombinant plasmids for gene expression analysis
• Performed RNA extraction, cDNA synthesis, qRT-PCR, and GUS staining to quantify and visualize gene expression in plant tissues

Bioinformatics Practical

• Utilized Ensembl genome browser to search gene of interest
• Used NCBI-BLAST and Linux/Unix to identify orthologs
• Constructed multiple sequence alignments using ClustalW to identify conserved amino acid regions
• Performed sequence analysis using ClustalW and InterProScan to identify conserved residues, predict functional protein domains, and annotate biological processes via Gene Ontology terms

Immunology Practical

• Used BD Accuri C6 software to analyze the flow cytometry assay