Tine Thoné
Antwerp
Summary
- Scientific curious and passionate QA Lab associate driven to contribute to development of high quality CAR-T cells for our patients. Highly motivated and demonstrated collaborative skills.
- Experience in coaching students and managing 1 direct report
- High level of expertise in immunology/oncology/hepB, and assay development for small & large molecules (incl. Bispecifics).
- Experience with PK/PD interpretation within the TLR8 program (writing of technical reports, publication).
- Experienced in coordination of studies, interpretation of data, presentation to key stakeholders, and troubleshooting.
- Eager to learn new aspects of drug development.
Overview
24
24
years of professional experience
Work History
QA Lab Ass. CAR-T
Johnson & Johnson
05.2024 - Current
- Spokesperson Critical Reagens
- SME role in flow cytometry & sterility testing
- Execute timely assigned periodic quality oversight activities such as quality tours, witnessing and audits
- Analyze timely and in depth investigations (LIR, NC), CAPA to ensure delivering qualitative products for patients
- Participate in TIER1/2 and actively work on good communication with supervisors, managers, trainers and analysts
- Review timely and efficiently GMP/GTP documents (procedures, protocols and reports)
- Take active role in problem solving
- Connect with different stakeholders, like method experts, critical reagens team, analysts, ELM team, investigator coordinators...
- Be a CAR-T ambassador by actively participating and presenting during connect moments (visits, townhalls, QC collaboration, quality month)
Scientist
Johnson & Johnson
10.2011 - 05.2024
- Succesfully led and organized Phase0 study: this study included evaluation of responses of 30 chronic HBV donors versus 20 healthy donors towards different TLR agonists.
- Wrote research papers, technical reports to support IB (including PK/PD), electronic labnote book reports.
- Supported early development team by generating data from in-licensed TLR compound. Presented data and conclusion to early development team.
- Collaborated with statisticians and programmers to streamline cytokine analysis from different platforms (ELISA, luminex, mesocale) (cytokine analysis tool).
- Collaborated with CRO's and gave input in design of experiments and followed up on results.
- Compared T-cell responses of chronic HBV patients with resolvers. This included troubleshooting of data generated by CRO, attending follow-up meetings, re-analysis of data together with statistical department, presenting findings to management.
- Assay-lead in bispecific program: development of killing assays; leading of colleague to evaluate binding of bispecifics on target cells.
- Developed different primary cellular assays to support candidate TLR (small molecule) agonist program.
- Trained new employees/students on areas of technical expertise and compliance issues relevant to lab setting.
- Member of TLR7/8 project team. Interactions with toxicologists, chemists, PK scientists.
Associate Scientist
Tibotec
03.2008 - 09.2011
- Developed IFN-alpha screening assay with primary blood cells to test activity of TLR agonists.
- Actively participated in finding alternatives to ELISA to detect IFN-alpha.
- Brought in expertise of working with primary cells and flow cytometry (peripheral blood mononuclear cells, PBMC).
- Developed primary cell-based assays to find and characterize TLR agonist small molecules.
- Scouted good source of human blood, organized blood supply for all Tibotec lab technicians.
- Remained up-to-date with flow cytometry: attended various courses.
- Documented changes during experiments, noting unexpected issues arising during research activities.
Researcher
Innogenetics/Genimmune
04.2001 - 02.2008
- Developed primary cell-based assay to evaluate tolerizing agens and adjuvants in dendritic cell-based assay (flow cytometry, ELISA)
- Compared responses between PBMC from Rheumatoid Arthritis patients versus PBMC from
healthy donors by ELISPOT after stimulation with various potential autoantigens (ELISPOT) - Set-up a collagen-induced arthritis model in mice
Education
FELASA C - Basic Course in Laboratory Animal Science
University of Ghent
2007
Master of Science - Cellular & Genetics Biotechnology, Bio-engineering
University of Ghent
2000
Erasmus Programme -
Swedish University of Agriculture
1999
Skills
- Handling LIRs and non-conformances
- GMP training at Vitalent
- Project management: Coordination and leading of in vitro studies, performing data analysis and presenting conducted studies, writing of technical reports of NME candidate
- Collaboration with cross-functional teams in or outside the organization
- Assay development with primary cells and cell lines, measuring responses towards small or large molecules: cytokine read-outs, CMV reactivation assay, flow cytometry assays to analyze responses of various subsets of immune cells, killing assays
- Analytical/critical thinking ; problem solving; interpreting data
- Open communication (including presenting data)
- Teamwork (building bridges) and connecting with people
- Taking initiative; coordinating and planning
- Continuous learning attitude
- Coaching / teaching
- Problem solving
- Scientific curiosity
- Good listener
- Flexible
Languages
Dutch
Bilingual or Proficient (C2)
English
Advanced (C1)
French
Intermediate (B1)
Publications
https://orcid.org/0000-0002-6698-8045
AL-034 (or TQ-A3334), a Selective Toll-like Receptor 7 Agonists for the Oral Treatment of Chronic Hepatitis B Virus Infection
[2017]
Poster @ AASLD by An De Creus
Profiling of a TLR agonist in human PBMC [2012]
Poster @ Knowledge for Growth (Ghent, Belgium)
Honors Awards/Training
- Pepal Leadership Training (2023): participated in a Leadership program in a team with people of Uganda and Janssen, to improve HIV awareness and reduce stigma amongst adolescents in the rural Kabwooya area.
- Award (2021): For the NME nomination of Janssen's novel TLR8 agonist.
- Award (2020): Setting up and validation of the immune checkpoint screen.
- Leadership Award (2019): For all the TLR in vitro work and development of the cytokine analysis tool.
- Award (2018): Implementation of the Cell Transfer Service together with Biobank Janssen Beerse.
- Award (2017): Support for the AL-034 studies: set up of all in vitro readouts, timely communication & analysis.
- Award (2015): Active collaboration and giving input in IMMO team meetings.
- Award (2014): Successful implementation of Human Blood Service for Beerse site.
Singing, Art, Nature
Singing in the local community choir; running; hiking; meeting friends and family; photography classes
Timeline
QA Lab Ass. CAR-T
Johnson & Johnson
05.2024 - Current
Scientist
Johnson & Johnson
10.2011 - 05.2024
Associate Scientist
Tibotec
03.2008 - 09.2011
Researcher
Innogenetics/Genimmune
04.2001 - 02.2008
Master of Science - Cellular & Genetics Biotechnology, Bio-engineering
University of Ghent
Erasmus Programme -
Swedish University of Agriculture
FELASA C - Basic Course in Laboratory Animal Science
University of Ghent
Similar Profiles
ANIS TRIKIANIS TRIKI
Medical Science Liaison – Urogenital Tumors at Johnson & Johnson Innovative MedicineMedical Science Liaison – Urogenital Tumors at Johnson & Johnson Innovative Medicine
Marzena KulisMarzena Kulis
VP, Strategy and Market Development EMEA at Johnson & Johnson MedTechVP, Strategy and Market Development EMEA at Johnson & Johnson MedTech
Russell Kyle KennedyRussell Kyle Kennedy
Warehouse Associate at Johnson & JohnsonWarehouse Associate at Johnson & Johnson
Salena BurroughsSalena Burroughs
Operations Recruitment Manager - Patient & Site Engagement at ICON Clinical Research (in partnership with Johnson & Johnson)Operations Recruitment Manager - Patient & Site Engagement at ICON Clinical Research (in partnership with Johnson & Johnson)
Radia BoumjimarRadia Boumjimar
Event Organizer at KukumariArtEvent Organizer at KukumariArt